Académicos del Departamento

Facultad de Medicina, UNAM

Dr. Oscar Flores Herrera

Laboratorio 3 6/4 (antes 4Bis), Edificio D, primer piso, Departamento de Bioquímica, Facultad de Medicina, UNAM
Tel. +52 (55) 56 23 25 10
BIOENERGETICA MITOCONDRIAL
Nuestro interés es sobre el estudio de la bioenergética mitocondrial en dos modelos: la placenta de humano, y el basidiomiceto Ustilago maydis. En los últimos años, nos hemos enfocado en el estudio de los supercomplejos de las mitocondrias de Ustilago maydis, particularmente los dímeros de la F1F0-ATP sintasa y los supercomplejos conformados por los complejos respiratorios I, III2 y IV. Se ha demostrado que los dímeros del complejo V participan en el plegamiento de las crastas mitocondriales. Respecto a los supercomplejos, su composición puede ser III2:IV, I:III2, o I:III2:IV siendo éstos últimos reconocidos como respirosomas, gracias a su capacidad de oxidar al NADH y reducir al oxígeno.
 

Hemos aislado estos supercomplejos y los hemos caracterizado cinéticamente. Ahora contamos con mutantes de las subunidades dimerizantes del complejo V y hemos iniciado el estudio de la repercusión de la eliminación de las subunidades dimerizantes en la estabilidad del dímero. Adicionalmente, estudiamos la arquitectura mitocondrial en las mutantes de las subunidades dimerizantes.

Experiencia Profesional

Trayectoria profesional

  • Investigador Titular “A” TC, SNI: I
  • Facultad de Medicina, UNAM (desde 1991)

Líneas de Investigación

  • Bioenergética mitocondrial; supercomplejos respiratorios

Formación académica

  • Doctorado en Investigación Biomédica Básica (2001), UNAM.
  • Maestría en Investigación Biomédica Básica (1996), UNAM.
  • Licenciatura en Biología, (1992), Facultad de Ciencias, UNAM.

Integrantes del laboratorio

  • Técnico Académico: Dra. Mercedes Esparza Perusquía

Alumnos

Giovanni García Cruz

Giovanni García Cruz

Estudiante de Licenciatura en Química, UNAM

Italo Güinno Lorandi Camacho

Italo Güinno Lorandi Camacho

Estudiante de Licenciatura en Biología, UNAM

Jaime Abraham de Lira Sánchez

Jaime Abraham de Lira Sánchez

Estudiante de Doctorado en Ciencias Biomedicas, UNAM

Stefanie Paola López Cervantes

Stefanie Paola López Cervantes

Estudiante de Licenciatura en Biología Experimental. UNAM-I

Publicaciones

Total sin duplicar: 502026 22025 32024 12023 42022 22021 32020 12019 32018 62017 22015 52014 12011 42010 22008 12006 12002 31999 31996 11995 11993 1

2026 (2)

  1. Rosa V, Rangel-Yescas GE, Flores-Herrera O, Esparza-Perusquia M, Cristóbal-Mondragón GR, Ramsey IS, Rosenbaum T, Islas LD. C-terminus-independent multimerization of Hv1 proton channels. The Journal of biological chemistry. 2026:113387. DOI · PubMed · PMID: 42551827
  2. Lorandi I, Hernández-Zúñiga JA, Esparza-Perusquía M, Matus-Ortega G, Vázquez-Meza H, Flores-Herrera H, Pardo JP, Martínez F, Flores-Herrera O. The internal alternative NADH dehydrogenase (Ndi1) is the electron input in the Saccharomyces cerevisiae respirasome. Biochimica et biophysica acta. Bioenergetics. 2026;1867(1):149574. DOI · PubMed · PMID: 41276210

2025 (3)

  1. Olvera-Sánchez S, Gómez-Chang E, Domínguez-López P, Flores-Herrera O, Martínez F. MAPK and PKA participate in the JEG-3 cell steroidogenesis. Biochemical and biophysical research communications. 2025;787:152796. DOI · PubMed · PMID: 41092728
  2. Sorescu JM, González-Montalvo MA, Yuan M, De Paolo-Boisvert J, Ceapă CD, Garcia-Contreras R, Flores-Herrera O, Shea ME, Tuz K, Juárez OX. Breakthroughs in the development of antibiotics, antifungals and antiparasitics targeting the pathogens' respiratory chain. Critical reviews in biochemistry and molecular biology. 2025;60(4-6):141-174. DOI · PubMed · PMID: 40844959
  3. García-Cruz G, Esparza-Perusquía M, Cruz-Cárdenas A, Cruz-Vilchis D, Flores-Herrera O. Kinetic characterization of respirasomes and free complex I from Yarrowia lipolytica. Mitochondrion. 2025;83:102035. DOI · PubMed · PMID: 40180170

2024 (1)

  1. López-Cervantes SP, Toledo-Pérez R, De Lira-Sánchez JA, García-Cruz G, Esparza-Perusquía M, Luna-López A, Pardo JP, Flores-Herrera O, Konigsberg M. Sedentary Lifestyles and a Hypercaloric Diets During Middle Age, are Binomial Conducive to Fatal Progression, That is Counteracted by the Hormetic Treatment of Exercise, Metformin, and Tert-Butyl Hydroquinone: An Analysis of Female Middle-Aged Rat Liver Mitochondria. Dose-response : a publication of International Hormesis Society. 2024;22(4):15593258241272619. DOI · PubMed · PMID: 39399210

2023 (4)

  1. de Lira-Sánchez JA, Esparza-Perusquía M, Martínez F, Pardo JP, Flores-Herrera O. Heavy metals do not induce ROS production by mitochondrial respirasome. Biochimica et biophysica acta. Bioenergetics. 2023;1864(4):148999. DOI · PubMed · PMID: 37516232
  2. Palacios-Luna JE, López-Marrufo MV, Bautista-Bautista G, Velarde-Guerra CS, Villeda-Gabriel G, Flores-Herrera O, Osorio-Caballero M, Aguilar-Carrasco JC, Palafox-Vargas ML, García-López G, Díaz-Ruíz O, Arechavaleta-Velasco F, et al.. Progesterone modulates extracellular heat-shock proteins and interlukin-1β in human choriodecidual after Escherichia coli infection. Placenta. 2023;142:85-94. DOI · PubMed · PMID: 37659254
  3. Bolaños-Suárez V, Alfaro A, Espinosa AM, Medina-Martínez I, Juárez E, Villegas-Sepúlveda N, Gudiño-Zayas M, Gutiérrez-Castro A, Román-Bassaure E, Salinas-Nieves ME, Bruno-Muñoz S, Aranda C, et al.. The mRNA and protein levels of the glycolytic enzymes lactate dehydrogenase A (LDHA) and phosphofructokinase platelet (PFKP) are good predictors of survival time, recurrence, and risk of death in cervical cancer patients. Cancer medicine. 2023;12(14):15632-15649. DOI · PubMed · PMID: 37326348
  4. Esparza-Perusquía M, Langner T, García-Cruz G, Feldbrügge M, Zavala G, Pardo JP, Martínez F, Flores-Herrera O. Deletion of the ATP20 gene in Ustilago maydis produces an unstable dimer of F(1)F(O)-ATP synthase associated with a decrease in mitochondrial ATP synthesis and a high H(2)O(2) production. Biochimica et biophysica acta. Bioenergetics. 2023;1864(2):148950. DOI · PubMed · PMID: 36509127

2022 (2)

  1. López-Cervantes SP, Sánchez NS, Calahorra M, Mena-Montes B, Pedraza-Vázquez G, Hernández-Álvarez D, Esparza-Perusquía M, Peña A, López-Díazguerrero NE, Alarcón-Aguilar A, Luna-López A, Flores-Herrera Ó, et al.. Moderate exercise combined with metformin-treatment improves mitochondrial bioenergetics of the quadriceps muscle of old female Wistar rats. Archives of gerontology and geriatrics. 2022;102:104717. DOI · PubMed · PMID: 35594738
  2. Pardo JP, Guerra-Sánchez G, Flores-Herrera O, Romero-Aguilar L. Isolation of Mitochondria from Ustilago maydis Protoplasts. Bio-protocol. 2022;12(1):e4277. DOI · PubMed · PMID: 35118170

2021 (3)

  1. Fuentes-Zacarias P, Arzate-Castañeda DA, Sosa-González I, Villeda-Gabriel G, Morales-Méndez I, Osorio-Caballero M, Helguera-Repetto AC, Díaz FN, García-López G, Flores-Herrera O, Arenas-Huertero F, Eslava-Campos C, et al.. Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1β, TNFα, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells. Acta biochimica Polonica. 2021;68(2):207-215. DOI · PubMed · PMID: 33945245
  2. Ruiz-Gómez X, Vázquez-Pérez JA, Flores-Herrera O, Esparza-Perusquía M, Santiago-Olivares C, Gaona-Bernal J, Gómez B, Mejía-Nepomuceno F, Méndez C, Rivera-Toledo E. Steady-state persistence of respiratory syncytial virus in a macrophage-like cell line and sequence analysis of the persistent viral genome. Virus research. 2021;297:198367. DOI · PubMed · PMID: 33684421
  3. Matuz-Mares D, Flores-Herrera O, Guerra-Sánchez G, Romero-Aguilar L, Vázquez-Meza H, Matus-Ortega G, Martínez F, Pardo JP. Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of Ustilago maydis Respiratory Supercomplexes. Journal of fungi (Basel, Switzerland). 2021;7(1). DOI · PubMed · PMID: 33440829

2020 (1)

  1. Romero-Aguilar L, Guerra-Sánchez G, Tenorio EP, Tapia-Rodriguez M, Matus-Ortega G, Flores-Herrera O, González J, Pardo JP. Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis. Archives of microbiology. 2020;202(5):1211-1221. DOI · PubMed · PMID: 32088730

2019 (3)

  1. Urban-Sosa VA, Olvera-Sánchez S, Barrera D, Aragón-Hernández JP, Flores-Herrera O, Martínez F. Isocitrate dehydrogenase type 2 (IDH2) is part of a multiprotein complex for placental steroidogenesis. Placenta. 2019;87:30-37. DOI · PubMed · PMID: 31542634
  2. Reyes-Galindo M, Suarez R, Esparza-Perusquía M, de Lira-Sánchez J, Pardo JP, Martínez F, Flores-Herrera O. Mitochondrial respirasome works as a single unit and the cross-talk between complexes I, III(2) and IV stimulates NADH dehydrogenase activity. Biochimica et biophysica acta. Bioenergetics. 2019;1860(8):618-627. DOI · PubMed · PMID: 31251900
  3. Ramírez-Camacho I, Flores-Herrera O, Zazueta C. The relevance of the supramolecular arrangements of the respiratory chain complexes in human diseases and aging. Mitochondrion. 2019;47:266-272. DOI · PubMed · PMID: 30664953

2018 (6)

  1. Ramírez-Camacho I, Correa F, El Hafidi M, Silva-Palacios A, Ostolga-Chavarría M, Esparza-Perusquía M, Olvera-Sánchez S, Flores-Herrera O, Zazueta C. Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts. Free radical biology & medicine. 2018;129:407-417. DOI · PubMed · PMID: 30316780
  2. Álvarez-Cabrera MC, Barrientos-Galeana E, Barrera-García A, Osorio-Caballero M, Acevedo JF, Flores-Herrera O, Díaz NF, Molina-Hernández A, García-López G, Flores-Herrera H. Secretion of heat shock -60, -70 kD protein, IL-1β and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development. Journal of cellular and molecular medicine. 2018;22(11):5748-5752. DOI · PubMed · PMID: 30133944
  3. Rendón JL, Miranda-Leyva M, Guevara-Flores A, Martínez-González JJ, Del Arenal IP, Flores-Herrera O, Pardo JP. Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR). Enzyme research. 2018;2018:3215462. DOI · PubMed · PMID: 30254758
  4. Sosa-Gutiérrez JA, Valdéz-Solana MA, Forbes-Hernández TY, Avitia-Domínguez CI, Garcia-Vargas GG, Salas-Pacheco JM, Flores-Herrera O, Téllez-Valencia A, Battino M, Sierra-Campos E. Effects of Moringa oleifera Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells. Biology. 2018;7(3). DOI · PubMed · PMID: 29949946
  5. Alejandra Sánchez-Muñoz M, Valdez-Solana MA, Campos-Almazán MI, Flores-Herrera Ó, Esparza-Perusquía M, Olvera-Sánchez S, García-Arenas G, Avitia-Domínguez C, Téllez-Valencia A, Sierra-Campos E. Streptozotocin-Induced Adaptive Modification of Mitochondrial Supercomplexes in Liver of Wistar Rats and the Protective Effect of Moringa oleifera Lam. Biochemistry research international. 2018;2018:5681081. DOI · PubMed · PMID: 29686903
  6. García-Gudiño I, Yllescas-Medrano E, Maida-Claros R, Soriano-Becerril D, Díaz NF, García-López G, Molina-Hernández A, Flores-Herrera O, Zavala-Díaz de la Serna FJ, Del Rosario Peralta-Pérez M, Flores-Herrera H. Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples. European journal of pediatrics. 2018;177(1):85-93. DOI · PubMed · PMID: 29090355

2017 (2)

  1. Esparza-Perusquía M, Olvera-Sánchez S, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O. Structural and kinetics characterization of the F(1)F(0)-ATP synthase dimer. New repercussion of monomer-monomer contact. Biochimica et biophysica acta. Bioenergetics. 2017;1858(12):975-981. DOI · PubMed · PMID: 28919501
  2. Guevara-Flores A, Herrera-Juárez ÁM, Martínez-González JJ, Del Arenal Mena IP, Flores-Herrera Ó, Rendón JL. Differential expression of disulfide reductase enzymes in a free-living platyhelminth (Dugesia dorotocephala). PloS one. 2017;12(8):e0182499. DOI · PubMed · PMID: 28787021

2015 (5)

  1. Martinez F, Olvera-Sanchez S, Esparza-Perusquia M, Gomez-Chang E, Flores-Herrera O. Multiple functions of syncytiotrophoblast mitochondria. Steroids. 2015;103:11-22. DOI · PubMed · PMID: 26435077
  2. Matus-Ortega MG, Cárdenas-Monroy CA, Flores-Herrera O, Mendoza-Hernández G, Miranda M, González-Pedrajo B, Vázquez-Meza H, Pardo JP. New complexes containing the internal alternative NADH dehydrogenase (Ndi1) in mitochondria of Saccharomyces cerevisiae. Yeast (Chichester, England). 2015;32(10):629-41. DOI · PubMed · PMID: 26173916
  3. Osorio-Caballero M, Perdigón-Palacio C, García-López G, Flores-Herrera O, Olvera-Sánchez S, Morales-Méndez I, Sosa-González I, Acevedo JF, Guzmán-Grenfell AM, Molina-Hernández A, Díaz NF, Flores-Herrera H. Escherichia coli-induced temporal and differential secretion of heat-shock protein 70 and interleukin-1β by human fetal membranes in a two-compartment culture system. Placenta. 2015;36(3):262-9. DOI · PubMed · PMID: 25600910
  4. Flores-Herrera O, Olvera-Sánchez S, Esparza-Perusquía M, Pardo JP, Rendón JL, Mendoza-Hernández G, Martínez F. Membrane potential regulates mitochondrial ATP-diphosphohydrolase activity but is not involved in progesterone biosynthesis in human syncytiotrophoblast cells. Biochimica et biophysica acta. 2015;1847(2):143-152. DOI · PubMed · PMID: 25444704
  5. Esparza-Perusquía M, Olvera-Sánchez S, Flores-Herrera O, Flores-Herrera H, Guevara-Flores A, Pardo JP, Espinosa-García MT, Martínez F. Mitochondrial proteases act on STARD3 to activate progesterone synthesis in human syncytiotrophoblast. Biochimica et biophysica acta. 2015;1850(1):107-17. DOI · PubMed · PMID: 25459514

2014 (1)

  1. Gomez-Chang E, Espinosa-Garcia MT, Olvera-Sanchez S, Flores-Herrera O, Martinez F. PKA tightly bound to human placental mitochondria participates in steroidogenesis and is not modified by cAMP. Placenta. 2014;35(9):748-62. DOI · PubMed · PMID: 25012296

2011 (4)

  1. Gomez-Concha C, Flores-Herrera O, Olvera-Sanchez S, Espinosa-Garcia MT, Martinez F. Progesterone synthesis by human placental mitochondria is sensitive to PKA inhibition by H89. The international journal of biochemistry & cell biology. 2011;43(9):1402-11. DOI · PubMed · PMID: 21689781
  2. Matus-Ortega MG, Salmerón-Santiago KG, Flores-Herrera O, Guerra-Sánchez G, Martínez F, Rendón JL, Pardo JP. The alternative NADH dehydrogenase is present in mitochondria of some animal taxa. Comparative biochemistry and physiology. Part D, Genomics & proteomics. 2011;6(3):256-63. DOI · PubMed · PMID: 21632289
  3. De los Rios Castillo D, Zarco-Zavala M, Olvera-Sanchez S, Pardo JP, Juarez O, Martinez F, Mendoza-Hernandez G, García-Trejo JJ, Flores-Herrera O. Atypical cristae morphology of human syncytiotrophoblast mitochondria: role for complex V. The Journal of biological chemistry. 2011;286(27):23911-9. DOI · PubMed · PMID: 21572045
  4. Olvera-Sanchez S, Espinosa-Garcia MT, Monreal J, Flores-Herrera O, Martinez F. Mitochondrial heat shock protein participates in placental steroidogenesis. Placenta. 2011;32(3):222-9. DOI · PubMed · PMID: 21232789

2010 (2)

  1. Milan R, Flores-Herrera O, Espinosa-Garcia MT, Olvera-Sanchez S, Martinez F. Contribution of potassium in human placental steroidogenesis. Placenta. 2010;31(10):860-6. DOI · PubMed · PMID: 20701969
  2. Guevara-Flores A, Del Arenal IP, Mendoza-Hernández G, Pardo JP, Flores-Herrera O, Rendón JL. Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci). Journal of parasitology research. 2010;2010. DOI · PubMed · PMID: 20798751

2008 (1)

  1. Guevara-Flores A, Olvera-Sánchez S, Gómez-Concha C, Juárez O, Esparza-Perusquía M, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O. 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci. Molecular and biochemical parasitology. 2008;162(2):123-33. DOI · PubMed · PMID: 18765260

2006 (1)

  1. Juárez O, Guerra G, Velázquez I, Flores-Herrera O, Rivera-Pérez RE, Pardo JP. The physiologic role of alternative oxidase in Ustilago maydis. The FEBS journal. 2006;273(20):4603-15. DOI · PubMed · PMID: 16965537

2002 (3)

  1. Flores-Herrera O, Uribe A, García-Pérez C, Milán R, Martínez F. 5'-p-Fluorosulfonylbenzoyl adenosine inhibits progesterone synthesis in human placental mitochondria. Biochimica et biophysica acta. 2002;1585(1):11-8. DOI · PubMed · PMID: 12457710
  2. Martínez F, Uribe A, Milán R, Teresa Espinosa-García M, Gracía-Pérez C, Flores-Herrera O. Differential effects of magnesium on the hydrolysis of ADP and ATP in human term placenta. Effect of substrates and potassium. The international journal of biochemistry & cell biology. 2002;34(8):1004-16. DOI · PubMed · PMID: 12007638
  3. Martínez F, Uribe A, Espinosa-García MT, Flores-Herrera O, García-Pérez C, Milán R. Calcium modulates the ATP and ADP hydrolysis in human placental mitochondria. The international journal of biochemistry & cell biology. 2002;34(8):992-1003. DOI · PubMed · PMID: 12007637

1999 (3)

  1. Flores-Herrera O, Uribe A, Pardo JP, Rendón JL, Martínez F. A novel ATP-diphosphohydrolase from human term placental mitochondria. Placenta. 1999;20(5-6):475-84. DOI · PubMed · PMID: 10419813
  2. Navarrete J, Flores-Herrera O, Uribe A, Martínez F. Differences in cholesterol incorporation into mitochondria from hepatoma AS-30D and human term placenta. Placenta. 1999;20(4):285-91. DOI · PubMed · PMID: 10329349
  3. Uribe A, Flores-Herrera O, Rendón JL, Espinosa-Garcia MT, Martinez F. Presence of two enzymes, different from the F1F0-ATPase, hydrolyzing nucleotides in human term placental mitochondria. The international journal of biochemistry & cell biology. 1999;31(2):319-30. DOI · PubMed · PMID: 10216964

1996 (1)

  1. Martínez F, Meaney A, Espinosa-García MT, Pardo JP, Uribe A, Flores-Herrera O. Characterization of the F1F0-ATPase and the tightly-bound ATPase activities in submitochondrial particles from human term placenta. Placenta. 1996;17(5-6):345-50. DOI · PubMed · PMID: 8829218

1995 (1)

  1. Martínez F, Pardo JP, Flores-Herrera O, Espinosa-García MT. The effect of osmolarity on human placental mitochondria function. The international journal of biochemistry & cell biology. 1995;27(8):795-803. DOI · PubMed · PMID: 7584614

1993 (1)

  1. Martínez F, Espinosa-García T, Flores-Herrera O, Pardo JP. Respiratory control induced by ATP in human term placental mitochondria. Placenta. 1993;14(3):321-31. DOI · PubMed · PMID: 8367413
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