Académicos del Departamento

Facultad de Medicina, UNAM

Dr. Oscar Flores Herrera

Laboratorio 3 6/4 (antes 4Bis), Edificio D, primer piso, Departamento de Bioquímica, Facultad de Medicina, UNAM
Tel. +52 (55) 56 23 25 10
BIOENERGETICA MITOCONDRIAL
Nuestro interés es sobre el estudio de la bioenergética mitocondrial en dos modelos: la placenta de humano, y el basidiomiceto Ustilago maydis. En los últimos años, nos hemos enfocado en el estudio de los supercomplejos de las mitocondrias de Ustilago maydis, particularmente los dímeros de la F1F0-ATP sintasa y los supercomplejos conformados por los complejos respiratorios I, III2 y IV. Se ha demostrado que los dímeros del complejo V participan en el plegamiento de las crastas mitocondriales. Respecto a los supercomplejos, su composición puede ser III2:IV, I:III2, o I:III2:IV siendo éstos últimos reconocidos como respirosomas, gracias a su capacidad de oxidar al NADH y reducir al oxígeno.
 

Hemos aislado estos supercomplejos y los hemos caracterizado cinéticamente. Ahora contamos con mutantes de las subunidades dimerizantes del complejo V y hemos iniciado el estudio de la repercusión de la eliminación de las subunidades dimerizantes en la estabilidad del dímero. Adicionalmente, estudiamos la arquitectura mitocondrial en las mutantes de las subunidades dimerizantes.

Experiencia Profesional

Trayectoria profesional

  • Investigador Titular “A” TC, SNI: I
  • Facultad de Medicina, UNAM (desde 1991)

Líneas de Investigación

  • Bioenergética mitocondrial; supercomplejos respiratorios

Formación académica

  • Doctorado en Investigación Biomédica Básica (2001), UNAM.
  • Maestría en Investigación Biomédica Básica (1996), UNAM.
  • Licenciatura en Biología, (1992), Facultad de Ciencias, UNAM.

Integrantes del laboratorio

  • Técnico Académico: Dra. Mercedes Esparza Perusquía

Alumnos

Giovanni García Cruz

Giovanni García Cruz

Estudiante de Licenciatura en Química, UNAM

Italo Güinno Lorandi Camacho

Italo Güinno Lorandi Camacho

Estudiante de Licenciatura en Biología, UNAM

Jaime Abraham de Lira Sánchez

Jaime Abraham de Lira Sánchez

Estudiante de Doctorado en Ciencias Biomedicas, UNAM

Stefanie Paola López Cervantes

Stefanie Paola López Cervantes

Estudiante de Licenciatura en Biología Experimental. UNAM-I

Publicaciones Académicas

53 publicaciones 1993 - 2025

2025

Aguilar LR, Sánchez GG, Herrera OF, Rendón JL, Meza HV, Pardo JP
Changes in macromolecular content and AOX activity of Ustilago maydis: from exponential to stationary phase.
Archives of microbiology (2025)
PubMed
PubMed DOI
Olvera-Sánchez S, Gómez-Chang E, Domínguez-López P, Flores-Herrera O, Martínez F
MAPK and PKA participate in the JEG-3 cell steroidogenesis.
Biochemical and biophysical research communications (2025)
PubMed
PubMed DOI
Sorescu JM, González-Montalvo MA, Yuan M, De Paolo-Boisvert J, Ceapă CD, Garcia-Contreras R, Flores-Herrera O, Shea ME, Tuz K, Juárez OX
Breakthroughs in the development of antibiotics, antifungals and antiparasitics targeting the pathogens' respiratory chain.
Critical reviews in biochemistry and molecular biology (2025)
PubMed
PubMed DOI
García-Cruz G, Esparza-Perusquía M, Cruz-Cárdenas A, Cruz-Vilchis D, Flores-Herrera O
Kinetic characterization of respirasomes and free complex I from Yarrowia lipolytica.
Mitochondrion (2025)
PubMed
PubMed DOI

2024

López-Cervantes SP, Toledo-Pérez R, De Lira-Sánchez JA, García-Cruz G, Esparza-Perusquía M, Luna-López A, Pardo JP, Flores-Herrera O, Konigsberg M
Sedentary Lifestyles and a Hypercaloric Diets During Middle Age, are Binomial Conducive to Fatal Progression, That is Counteracted by the Hormetic Treatment of Exercise, Metformin, and Tert-Butyl Hydroquinone: An Analysis of Female Middle-Aged Rat Liver Mitochondria.
Dose-response : a publication of International Hormesis Society (2024)
PubMed
PubMed DOI

2023

Palacios-Luna JE, López-Marrufo MV, Bautista-Bautista G, Velarde-Guerra CS, Villeda-Gabriel G, Flores-Herrera O, Osorio-Caballero M, Aguilar-Carrasco JC, Palafox-Vargas ML, García-López G, Díaz-Ruíz O, Arechavaleta-Velasco F, Flores-Herrera H
Progesterone modulates extracellular heat-shock proteins and interlukin-1β in human choriodecidual after Escherichia coli infection.
Placenta (2023)
PubMed
PubMed DOI
de Lira-Sánchez JA, Esparza-Perusquía M, Martínez F, Pardo JP, Flores-Herrera O
Heavy metals do not induce ROS production by mitochondrial respirasome.
Biochimica et biophysica acta. Bioenergetics (2023)
PubMed
PubMed DOI
Bolaños-Suárez V, Alfaro A, Espinosa AM, Medina-Martínez I, Juárez E, Villegas-Sepúlveda N, Gudiño-Zayas M, Gutiérrez-Castro A, Román-Bassaure E, Salinas-Nieves ME, Bruno-Muñoz S, Aranda C, Flores-Herrera O, Berumen J
The mRNA and protein levels of the glycolytic enzymes lactate dehydrogenase A (LDHA) and phosphofructokinase platelet (PFKP) are good predictors of survival time, recurrence, and risk of death in cervical cancer patients.
Cancer medicine (2023)
PubMed
PubMed DOI
Esparza-Perusquía M, Langner T, García-Cruz G, Feldbrügge M, Zavala G, Pardo JP, Martínez F, Flores-Herrera O
Deletion of the ATP20 gene in Ustilago maydis produces an unstable dimer of FF-ATP synthase associated with a decrease in mitochondrial ATP synthesis and a high HO production.
Biochimica et biophysica acta. Bioenergetics (2023)
PubMed
PubMed DOI

2022

López-Cervantes SP, Sánchez NS, Calahorra M, Mena-Montes B, Pedraza-Vázquez G, Hernández-Álvarez D, Esparza-Perusquía M, Peña A, López-Díazguerrero NE, Alarcón-Aguilar A, Luna-López A, Flores-Herrera Ó, Königsberg M
Moderate exercise combined with metformin-treatment improves mitochondrial bioenergetics of the quadriceps muscle of old female Wistar rats.
Archives of gerontology and geriatrics (2022)
PubMed
PubMed DOI
Pardo JP, Guerra-Sánchez G, Flores-Herrera O, Romero-Aguilar L
Isolation of Mitochondria from Protoplasts.
Bio-protocol (2022)
PubMed
PubMed DOI

2021

Fuentes-Zacarias P, Arzate-Castañeda DA, Sosa-González I, Villeda-Gabriel G, Morales-Méndez I, Osorio-Caballero M, Helguera-Repetto AC, Díaz FN, García-López G, Flores-Herrera O, Arenas-Huertero F, Eslava-Campos C, Díaz-Ruíz O, Flores-Herrera H
Pseudomonas aeruginosa induces spatio-temporal secretion of IL-1β, TNFα, proMMP-9, and reduction of epithelial E-cadherin in human alveolar epithelial type II (A549) cells.
Acta biochimica Polonica (2021)
PubMed
PubMed DOI
Lucero RA, Mercedes EP, Thorsten L, Giovanni GC, Michael F, Guadalupe Z, Pablo PJ, Federico M, Oscar FH
Deletion of the natural inhibitory protein Inh1 in Ustilago maydis has no effect on the dimeric state of the FF-ATP synthase but increases the ATPase activity and reduces the stability.
Biochimica et biophysica acta. Bioenergetics (2021)
PubMed
PubMed DOI
Ruiz-Gómez X, Vázquez-Pérez JA, Flores-Herrera O, Esparza-Perusquía M, Santiago-Olivares C, Gaona-Bernal J, Gómez B, Mejía-Nepomuceno F, Méndez C, Rivera-Toledo E
Steady-state persistence of respiratory syncytial virus in a macrophage-like cell line and sequence analysis of the persistent viral genome.
Virus research (2021)
PubMed
PubMed DOI
Matuz-Mares D, Flores-Herrera O, Guerra-Sánchez G, Romero-Aguilar L, Vázquez-Meza H, Matus-Ortega G, Martínez F, Pardo JP
Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of Respiratory Supercomplexes.
Journal of fungi (Basel, Switzerland) (2021)
PubMed
PubMed DOI

2020

Romero-Aguilar L, Guerra-Sánchez G, Tenorio EP, Tapia-Rodriguez M, Matus-Ortega G, Flores-Herrera O, González J, Pardo JP
Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.
Archives of microbiology (2020)
PubMed
PubMed DOI

2019

Urban-Sosa VA, Olvera-Sánchez S, Barrera D, Aragón-Hernández JP, Flores-Herrera O, Martínez F
Isocitrate dehydrogenase type 2 (IDH2) is part of a multiprotein complex for placental steroidogenesis.
Placenta (2019)
PubMed
PubMed DOI
Reyes-Galindo M, Suarez R, Esparza-Perusquía M, de Lira-Sánchez J, Pardo JP, Martínez F, Flores-Herrera O
Mitochondrial respirasome works as a single unit and the cross-talk between complexes I, III and IV stimulates NADH dehydrogenase activity.
Biochimica et biophysica acta. Bioenergetics (2019)
PubMed
PubMed DOI
Ramírez-Camacho I, Flores-Herrera O, Zazueta C
The relevance of the supramolecular arrangements of the respiratory chain complexes in human diseases and aging.
Mitochondrion (2019)
PubMed
PubMed DOI

2018

Rendón JL, Miranda-Leyva M, Guevara-Flores A, Martínez-González JJ, Del Arenal IP, Flores-Herrera O, Pardo JP
Insight into the Mechanistic Basis of the Hysteretic-Like Kinetic Behavior of Thioredoxin-Glutathione Reductase (TGR).
Enzyme research (2018)
PubMed
PubMed DOI
Álvarez-Cabrera MC, Barrientos-Galeana E, Barrera-García A, Osorio-Caballero M, Acevedo JF, Flores-Herrera O, Díaz NF, Molina-Hernández A, García-López G, Flores-Herrera H
Secretion of heat shock -60, -70 kD protein, IL-1β and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development.
Journal of cellular and molecular medicine (2018)
PubMed
PubMed DOI
Sosa-Gutiérrez JA, Valdéz-Solana MA, Forbes-Hernández TY, Avitia-Domínguez CI, Garcia-Vargas GG, Salas-Pacheco JM, Flores-Herrera O, Téllez-Valencia A, Battino M, Sierra-Campos E
Effects of Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells.
Biology (2018)
PubMed
PubMed DOI
Alejandra Sánchez-Muñoz M, Valdez-Solana MA, Campos-Almazán MI, Flores-Herrera Ó, Esparza-Perusquía M, Olvera-Sánchez S, García-Arenas G, Avitia-Domínguez C, Téllez-Valencia A, Sierra-Campos E
Streptozotocin-Induced Adaptive Modification of Mitochondrial Supercomplexes in Liver of Wistar Rats and the Protective Effect of Lam.
Biochemistry research international (2018)
PubMed
PubMed DOI
García-Gudiño I, Yllescas-Medrano E, Maida-Claros R, Soriano-Becerril D, Díaz NF, García-López G, Molina-Hernández A, Flores-Herrera O, Zavala-Díaz de la Serna FJ, Del Rosario Peralta-Pérez M, Flores-Herrera H
Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples.
European journal of pediatrics (2018)
PubMed
PubMed DOI
Ramírez-Camacho I, Correa F, El Hafidi M, Silva-Palacios A, Ostolga-Chavarría M, Esparza-Perusquía M, Olvera-Sánchez S, Flores-Herrera O, Zazueta C
Cardioprotective strategies preserve the stability of respiratory chain supercomplexes and reduce oxidative stress in reperfused ischemic hearts.
Free radical biology & medicine (2018)
PubMed
PubMed DOI

2017

Esparza-Perusquía M, Olvera-Sánchez S, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O
Structural and kinetics characterization of the FF-ATP synthase dimer. New repercussion of monomer-monomer contact.
Biochimica et biophysica acta. Bioenergetics (2017)
PubMed
PubMed DOI
Guevara-Flores A, Herrera-Juárez ÁM, Martínez-González JJ, Del Arenal Mena IP, Flores-Herrera Ó, Rendón JL
Differential expression of disulfide reductase enzymes in a free-living platyhelminth (Dugesia dorotocephala).
PloS one (2017)
PubMed
PubMed DOI

2015

Martinez F, Olvera-Sanchez S, Esparza-Perusquia M, Gomez-Chang E, Flores-Herrera O
Multiple functions of syncytiotrophoblast mitochondria.
Steroids (2015)
PubMed
PubMed DOI
Esparza-Perusquía M, Olvera-Sánchez S, Flores-Herrera O, Flores-Herrera H, Guevara-Flores A, Pardo JP, Espinosa-García MT, Martínez F
Mitochondrial proteases act on STARD3 to activate progesterone synthesis in human syncytiotrophoblast.
Biochimica et biophysica acta (2015)
PubMed
PubMed DOI
Flores-Herrera O, Olvera-Sánchez S, Esparza-Perusquía M, Pardo JP, Rendón JL, Mendoza-Hernández G, Martínez F
Membrane potential regulates mitochondrial ATP-diphosphohydrolase activity but is not involved in progesterone biosynthesis in human syncytiotrophoblast cells.
Biochimica et biophysica acta (2015)
PubMed
PubMed DOI
Matus-Ortega MG, Cárdenas-Monroy CA, Flores-Herrera O, Mendoza-Hernández G, Miranda M, González-Pedrajo B, Vázquez-Meza H, Pardo JP
New complexes containing the internal alternative NADH dehydrogenase (Ndi1) in mitochondria of Saccharomyces cerevisiae.
Yeast (Chichester, England) (2015)
PubMed
PubMed DOI
Osorio-Caballero M, Perdigón-Palacio C, García-López G, Flores-Herrera O, Olvera-Sánchez S, Morales-Méndez I, Sosa-González I, Acevedo JF, Guzmán-Grenfell AM, Molina-Hernández A, Díaz NF, Flores-Herrera H
Escherichia coli-induced temporal and differential secretion of heat-shock protein 70 and interleukin-1β by human fetal membranes in a two-compartment culture system.
Placenta (2015)
PubMed
PubMed DOI

2014

Gomez-Chang E, Espinosa-Garcia MT, Olvera-Sanchez S, Flores-Herrera O, Martinez F
PKA tightly bound to human placental mitochondria participates in steroidogenesis and is not modified by cAMP.
Placenta (2014)
PubMed
PubMed DOI

2013

Robles-Martínez L, Guerra-Sánchez MG, Flores-Herrera O, Hernández-Lauzardo AN, Velázquez-Del Valle MG, Pardo JP
The mitochondrial respiratory chain of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill.
Archives of microbiology (2013)
PubMed
PubMed DOI

2011

Gomez-Concha C, Flores-Herrera O, Olvera-Sanchez S, Espinosa-Garcia MT, Martinez F
Progesterone synthesis by human placental mitochondria is sensitive to PKA inhibition by H89.
The international journal of biochemistry & cell biology (2011)
PubMed
PubMed DOI
Matus-Ortega MG, Salmerón-Santiago KG, Flores-Herrera O, Guerra-Sánchez G, Martínez F, Rendón JL, Pardo JP
The alternative NADH dehydrogenase is present in mitochondria of some animal taxa.
Comparative biochemistry and physiology. Part D, Genomics & proteomics (2011)
PubMed
PubMed DOI
De los Rios Castillo D, Zarco-Zavala M, Olvera-Sanchez S, Pardo JP, Juarez O, Martinez F, Mendoza-Hernandez G, García-Trejo JJ, Flores-Herrera O
Atypical cristae morphology of human syncytiotrophoblast mitochondria: role for complex V.
The Journal of biological chemistry (2011)
PubMed
PubMed DOI
Salmerón-Santiago KG, Pardo JP, Flores-Herrera O, Mendoza-Hernández G, Miranda-Arango M, Guerra-Sánchez G
Response to osmotic stress and temperature of the fungus Ustilago maydis.
Archives of microbiology (2011)
PubMed
PubMed DOI
Olvera-Sanchez S, Espinosa-Garcia MT, Monreal J, Flores-Herrera O, Martinez F
Mitochondrial heat shock protein participates in placental steroidogenesis.
Placenta (2011)
PubMed
PubMed DOI

2010

Guevara-Flores A, Del Arenal IP, Mendoza-Hernández G, Pardo JP, Flores-Herrera O, Rendón JL
Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci).
Journal of parasitology research (2010)
PubMed
PubMed DOI
Milan R, Flores-Herrera O, Espinosa-Garcia MT, Olvera-Sanchez S, Martinez F
Contribution of potassium in human placental steroidogenesis.
Placenta (2010)
PubMed
PubMed DOI

2008

Guevara-Flores A, Olvera-Sánchez S, Gómez-Concha C, Juárez O, Esparza-Perusquía M, Pardo JP, Mendoza-Hernández G, Martínez F, Flores-Herrera O
5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP-diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci.
Molecular and biochemical parasitology (2008)
PubMed
PubMed DOI

2006

Juárez O, Guerra G, Velázquez I, Flores-Herrera O, Rivera-Pérez RE, Pardo JP
The physiologic role of alternative oxidase in Ustilago maydis.
The FEBS journal (2006)
PubMed
PubMed

2002

Martínez F, Uribe A, Milán R, Teresa Espinosa-García M, Gracía-Pérez C, Flores-Herrera O
Differential effects of magnesium on the hydrolysis of ADP and ATP in human term placenta. Effect of substrates and potassium.
The international journal of biochemistry & cell biology (2002)
PubMed
PubMed
Martínez F, Uribe A, Espinosa-García MT, Flores-Herrera O, García-Pérez C, Milán R
Calcium modulates the ATP and ADP hydrolysis in human placental mitochondria.
The international journal of biochemistry & cell biology (2002)
PubMed
PubMed
Flores-Herrera O, Uribe A, García-Pérez C, Milán R, Martínez F
5'-p-Fluorosulfonylbenzoyl adenosine inhibits progesterone synthesis in human placental mitochondria.
Biochimica et biophysica acta (2002)
PubMed
PubMed

1999

Flores-Herrera O, Uribe A, Pardo JP, Rendón JL, Martínez F
A novel ATP-diphosphohydrolase from human term placental mitochondria.
Placenta (1999)
PubMed
PubMed
Navarrete J, Flores-Herrera O, Uribe A, Martínez F
Differences in cholesterol incorporation into mitochondria from hepatoma AS-30D and human term placenta.
Placenta (1999)
PubMed
PubMed
Uribe A, Flores-Herrera O, Rendón JL, Espinosa-Garcia MT, Martinez F
Presence of two enzymes, different from the F1F0-ATPase, hydrolyzing nucleotides in human term placental mitochondria.
The international journal of biochemistry & cell biology (1999)
PubMed
PubMed

1996

Martínez F, Meaney A, Espinosa-García MT, Pardo JP, Uribe A, Flores-Herrera O
Characterization of the F1F0-ATPase and the tightly-bound ATPase activities in submitochondrial particles from human term placenta.
Placenta (1996)
PubMed
PubMed

1995

Martínez F, Pardo JP, Flores-Herrera O, Espinosa-García MT
The effect of osmolarity on human placental mitochondria function.
The international journal of biochemistry & cell biology (1995)
PubMed
PubMed
Flores-Herrera O, Pardo JP, Espinosa-García MT, Martínez F
Calcium transport in human term placental mitochondria.
Biochemistry and molecular biology international (1995)
PubMed
PubMed

1993

Martínez F, Espinosa-García T, Flores-Herrera O, Pardo JP
Respiratory control induced by ATP in human term placental mitochondria.
Placenta (1993)
PubMed
PubMed

Estadísticas

Total de Publicaciones
53
Periodo
1993 - 2025

Temas Principales

Humans (28) Mitochondria (27) Female (22) Pregnancy (18) Placenta (15) Progesterone (12) Adenosine triphosphate (11) Reactive oxygen species (8) Kinetics (8) Animals (8) Oxygen consumption (8) Trophoblasts (7) Ustilago (6) Oxidative phosphorylation (6) Mitochondrial proteins (5)
Idioma »
error: Contenido Protegido; Departamento de Bioquímica, Facultad de Medicna, UNAM